Composite

Part:BBa_K2844011:Design

Designed by: Morganne Wilbourne   Group: iGEM18_St_Andrews   (2018-10-09)


codon optimized mNeonGreen with promoter, RBS, and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used this for the part improvement component of our project, as the sequence is different from the mNeonGreen currently in the registry.


Source

mNeonGreen comes from Branchiosoma lanceolatum

References